PhD, Stanford University
Postdoctoral, Cornell University
Office: Thomas Hall
Website: Visit our Lab Home Page
Research Areas: Computation / Bioinformatics | Population / Quantitative
My research uses laboratory, bioinformatic, and comparative genomic tools to illuminate the individual and joint contributions of various evolutionary forces to patterns of genome evolution, such that the underlying causes of molecular change can be inferred. Currently, my work focuses on understanding the spatial and temporal scales over which mutation rate and recombination rate are variable, using Drosophila as a model system. Mutation rate variation can be assessed using patterns of polymorphism and divergence from neutrally evolving regions of the genome; previous studies of this nature have revealed remarkable heterogeneity in molecular evolutionary patterns both within and among Drosophila genomes. Intragenomic recombination rate variation can also be inferred using patterns of polymorphism, and the availability of full genome sequences from a population sample of D. melanogaster presents an exciting opportunity for this avenue of research. Variation in recombination rate can also be measured experimentally, using classical genetic approaches. A major focus of the lab is characterizing the fine-scale structure of crossover distribution in a small region of the D. melanogaster X chromosome using a classical genetic approach coupled with next-generation sequencing, and examining how this structure varies within a population.
Singh ND, Aquadro CF, Clark AG (2009) Estimation of fine-scale recombination intensity variation in the white-echinus region of D. melanogaster. Journal of Molecular Evolution 69:42-53.
Singh ND, Arndt PF, Clark AG, Aquadro CF (2009) Strong evidence for lineage- and sequence-specificity of substitution rates and patterns in Drosophila. Molecular Biology and Evolution 26:1591-1605.
Singh ND, Larracuente AM, Clark AG (2008) Contrasting the efficacy of selection on the X and autosomes in Drosophila. Molecular Biology and Evolution 25:454-467.
Singh ND, Macpherson JM, Jensen JD, Petrov DA (2007) Similar levels of X-linked and autosomal nucleotide polymorphism in African and non-African strains of Drosophila melanogaster. BMC Evolutionary Biology 7:202.
Singh ND, Davis JC, Petrov DA (2005) X-linked genes evolve higher codon bias in Drosophila and Caenorhabditis. Genetics 171:145-155.
Singh ND, Petrov DA (2004) Rapid sequence turnover at an intergenic locus in Drosophila. Molecular Biology and Evolution 21:670-680.