North Carolina State University - Department Of Genetics

Trudy F. C. Mackay
Trudy F. C. Mackay
Program in Genetics

People

William Neal Reynolds Distinguished University Professor of Biological Sciences and Associate Member of Entomology

Type: Faculty

Education:
PhD, University of Edinburgh
Postdoctoral, Dalhousie University

Contact Info
Office: 3550 Thomas Hall, 919-515-5810
Lab: 3555 Thomas Hall, 919-515-5833
Email: Trudy_Mackay

Website: Visit our Lab Home Page

Research Areas: Behavioral / Biomedical | Population / Quantitative

My research focuses on understanding the genetic and environmental factors affecting variation in quantitative traits, using Drosophila as a model system. Understanding of the genetic architecture of quantitative traits requires that we know the genetic loci at which segregating and mutational variation occurs; the homozygous, heterozygous and epistatic allelic effects, pleiotropic effects on other characters, including fitness; environmental sensitivities of QTL alleles; and the molecular genetic basis of quantitative variation in nature. My lab uses several complementary approaches to achieve these goals. We screen P-element insertion mutations to identify candidate genes and pathways. We map QTLs by linkage to polymorphic molecular markers in crosses between genetically divergent strains, followed by complementation tests to deficiencies for high resolution mapping and to mutations to identify candidate genes. We also map QTLs by associating molecular polymorphisms with quantitative phenotypes on a genome wide scale using our Drosophila Genetic Reference Panel of 192 inbred lines, for which complete genome sequence will be available in the near future. Since DNA polymorphisms affect variation in quantitative traits by perturbing transcripts, metabolites and proteins, we are incorporating variation in transcript abundance in these studies, to provide biological context to the QTLs and identify transcriptional and genetic networks affecting complex traits. The effects of QTL alleles can also be environment-specific; therefore, we incorporate ecologically relevant environments in the above studies. We perform these studies on morphological (sensory bristle number); behavioral (olfaction, locomotion, aggression, mating, alcohol sensitivity, sleep) and life history (longevity, resistance to starvation, heat, cold and oxidative stress) traits in D. melanogaster; and on morphological (pigmentation) and behavioral (mating) divergence between closely related species pairs (D. mauritiana/D. simulans and D. yakuba/D. santomea). Understanding the genetic architecture of Drosophila quantitative traits is necessary to understand the evolutionary forces causing variation for quantitative traits within populations and phenotypic divergence between populations and species. General principles are likely to be conserved in other species, including humans. Finally, the same genes and networks affecting complex traits that are orthologous between flies and humans may be conserved; an hypothesis that we are directly testing for several traits.

Selected Publications:

Ayroles J F, Carbone MA, Stone EA, Jordan KW, Lyman RF, Magwire MM, Rollmann SM, Duncan LH, Lawrence F, Anholt RRH, and Mackay TFC. (2009). Systems genetics of complex traits in Drosophila melanogaster. Nat. Genet. 41: 299–307. PMCID: PMC2752214.

Edwards AC, Ayroles JF, Stone EA, Carbone MA, Lyman RF, and Mackay TFC. (2009). A transcriptional network associated with natural variation in Drosophila aggressive behavior. Genome Biology. 10: R76. PMCID: PMC2728530.

Harbison ST, Carbone MA, Ayroles JF, Stone EA, Lyman RF, and Mackay TFC. (2009). Co-regulated transcriptional networks contribute to natural genetic variation in Drosophila sleep. Nat. Genet. 41: 371–375. PMCID: PMC2683981

Mackay TFC, Stone EA, and Ayroles JF. (2009). The genetics of quantitative traits: Challenges and prospects. Nat. Rev. Genet. 10: 565–577.

Morozova TV, Ayroles JF, Jordan KW, Duncan LH, Carbone MA, Lyman RF, Stone EA, Govindaraju DR, Ellison CR, Mackay TFC, and Anholt RRH. (2009). Alcohol sensitivity in Drosophila: Translational potential of systems genetics. Genetics. 83: 733–745. PMCID: PMC2766331.

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Program in Genetics
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N.C. State University
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